High concentrations of aldehydes slow the reaction of cytoplasmic aldehyde dehydrogenase with thiol-group modifiers.
نویسنده
چکیده
High concentrations of aldehydes slow the inactivation of cytoplasmic aldehyde dehydrogenase by disulfiram and also slow the reaction of the enzyme with 2,2'-dithiodipyridine. It is concluded that a low-affinity aldehyde-binding site is probably the site at which thiol-group modifiers react with aldehyde dehydrogenase, as well as being the active site for hydrolysis of 4-nitrophenyl acetate.
منابع مشابه
Effects of aliphatic aldehyde metabolism on protein synthesis and thiol compounds in rat liver and hepatoma induced by 4-dimethylaminoazobenzene.
The effects of isobutyraldehyde and 2,3-dihydroxybutyraldehyde on protein synthesis, adenosine triphosphate, nonprotein sulfhydryl compounds, and glutathione levels, as well as the metabolic rate of some aliphatic aldehydes, were studied in slices of rat liver and hepatoma induced by 4dimethylaminoazobenzene. Aliphatic aldehydes depressed protein synthesis in liver and hepatoma cells but not in...
متن کامل1,2-sulfanyl group migration as a driving force: new approach to pyrroles by reaction of allenic aldehydes with amines.
[reaction: see text] Acid-promoted reaction of sulfanyl group substituted allenic aldehyde with amine affords pyrrole derivatives in high yields. The neighboring group participation of the sulfanyl group is the driving force in this transformation.
متن کاملThe mechanism of oxidation of aldehydes by glyceralde-hyde-3-phosphate dehydrogenase.
It has been demonstrated (1) that the oxidation-reduction of methylglyoxal to lactic acid occurs in two steps. The first step is a condensation of methylglyoxal and reduced glutathione to a thiol ester which is hydrolytically cleaved to lactic acid and reduced glutathione. It was pointed out that such a mechanism might be operative in other aldehyde-oxidizing systems such as glyceraldehyde phos...
متن کاملAldehyde Dehydrogenase: Cancer and Stem Cells
Aldehyde dehydrogenases (ALDH) belong to the oxidoreductase family, which catalyze the conversion of aldehydes to their corresponding acids. As a group of NAD(P)+-dependent enzymes, aldehyde dehydrogenases (ALDHs) are involved in oxidation of a large number of aldehydes into their weak carboxylic acids (Moreb, et al., 2012). ALDH is found in every subcellular region such as cytosol, endoplasmic...
متن کاملKinetic properties of highly purified preparations of sheep liver cytoplasmic aldehyde dehydrogenase.
The kinetic properties of highly purified preparations of sheep liver cytoplasmic aldehyde dehydrogenase (preparations that had been shown to be free from contamination with the corresponding mitochondrial enzyme) were investigated with both propionaldehyde and butyraldehyde as substrates. At low aldehyde concentrations, double-reciprocal plots with aldehyde as the variable substrate are linear...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Biochemical journal
دوره 228 3 شماره
صفحات -
تاریخ انتشار 1985